Is there any relationship between Chlamydophila pneumoniae and coronary atherosclerosis among Iranians?
Mohammad Hadi Sadeghian1, Seyed Abbas Tabatabaee Yazdi2, Hossein Ayatollahi1, Mohammad Reza Keramati3, Kiarash Ghazvini4, Ali Reza Rezai5, Nasrin Heidari6, Maryam Sheikhi1, Gohar Shaghayegh1
1 Faculty of Medicine, Cancer Molecular Pathology Research Centre, Ghaem Medical Centre, Mashhad, Iran
2 Department of Pathology, Ghaem Medical Centre, Mashhad, Iran
3 Neonatal Research Centre, Faculty of Medicine, Imam Reza Medical Centre, Mashhad, Iran
4 Department of Microbiology, Ghaem Medical Centre, Mashhad, Iran
5 Department of Hematology and Blood Bank, Ghaem Medical Centre, Mashhad, Iran
6 Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Cancer Molecular Pathology Research Centre, Ghaem Medical Center, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad
Source of Support: Research Vice Chancellor of Mashhad University of Medical Sciences,, Conflict of Interest: None
Background: Atherosclerosis is a coronary heart disease, andis the most common cause of death in the industrialized world. Some studies suggested that atherosclerosis may be triggered by infectious agents, mostly Chlamydophila pneumoniae. However, the role of C. pneumoniae in the pathogenesis of coronary atherosclerosis is still controversial. Objectives: This study was performed to evaluate whether there is a significant association between coronary artery atherosclerosis and C. pneumoniae by the polymerase chain reaction (PCR) method. Materials and Methods: This case-control study was carried out on formalin-fixed paraffin-embedded tissue biopsies of the coronary arteries obtained from 30 patients with coronary atherosclerosis and 30 subjects without atherosclerosis living in Northeast of Iran. All subjects' weight and height were determined, and the body mass index was calculated. We also reviewed the medical history and previous laboratory reports of patients. Deoxyribonucleic acid (DNA) was extracted, and C. pneumonia DNA was amplified and detected using PCR assay. Results: The age of the patients in the study group was from 18 to 50 years, and the male to female ratio was 5:1. Only oneout of the 30 coronary tissue samples had positive PCR for C. pneumoniae (3.3%), while it was negative for patients in the control group. Conclusions: This study showed that C. pneumoniae infection is not strongly associated with coronary artery atherosclerosis in Northeast of Iran.